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Antimicrobial Agents and Chemotherapy
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Antimicrobial Agents and Chemotherapy
Article . 1978 . Peer-reviewed
License: ASM Journals Non-Commercial TDM
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Antimicrobial Agents and Chemotherapy
Article . 1978
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https://dx.doi.org/10.1128/aac...
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Beta-Lactamases: Determination of Their Isoelectric Points

descriptionPublicationkeyboard_double_arrow_right Article 01 Apr 1978 English Publisher:American Society for MicrobiologyJournal:Antimicrobial Agents and Chemotherapy, volume 13, pages 695-698 (issn: 0066-4804, eissn: 1098-6596, Copyright policy )
Authors: M, Barthelemy; M, Guionie; R, Labia;

doi: 10.1128/aac.13.4.695

pmid: 96731

pmc: PMC352312

Beta-Lactamases: Determination of Their Isoelectric Points

Abstract

Important discrepancies in isoelectric points (pI) of β-lactamases were observed depending on the experimental procedure used for their determination: isoelectric focusing (IEF) in sucrose density gradients or analytical IEF in thin-layer polyacrylamide gels (PA). The variations, negligable in the case of TEM-like β-lactamases, appeared to be important in the case of cephalosporinases and are related to an artifact which appears in PA-IEF. This has been clearly shown with β-lactamase preparations from the following bacterial strains: Pseudomonas aeruginosa NCTC 8203 (pI = 8.7), P. aeruginosa NCTC 10701 (pI = 9.4), and Proteus morganii NCTC 235 (pI = 8.3). The data previously obtained by PA-IEF were much lower.

Related Organizations
Keywords

Molecular Weight, Pseudomonas aeruginosa, Escherichia coli, Isoelectric Point, Isoelectric Focusing, Amidohydrolases, Cephalosporinase

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    selected citations
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    This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
    		 75
    popularity
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    		 Top 10%
    influence
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    impulse
    This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
75
Top 10%
Top 1%
Top 10%
bronze