AbstractPurposeTo determine the significance ofAspergillusandFusariumspp., as identified by culture, on clinical outcome in equine keratomycosis.MethodsRetrospective analysis of 66 horses (66 eyes) evaluated at the NCSU‐VH diagnosed with keratomycosis from whichAspergillusorFusariumspp. were cultured. Horses were classified into those who improved with medical management alone or those who required surgical intervention to improve. Horses who underwent surgery were divided into globe‐sparing procedures or enucleation. Effects of bacterial co‐infection, previous topical steroid or antifungal use, and time of year on fungal genus and outcome were evaluated.ResultsAspergillusspp. was cultured from 41 eyes (63%), while 24 eyes (37%) culturedFusariumspp. One horse cultured both species and was not included in further evaluation. From the horses that culturedAspergillusspp., 28 eyes (68%) required surgical intervention to control the infection: 21 (75%) of these eyes maintained globe integrity, while 7 eyes (25%) were enucleated. Of those horses withFusariumspp., 14 eyes (58%) required surgical intervention: 11 (79%) of these eyes maintained globe integrity, while 3 eyes (21%) were enucleated. Genus of fungus cultured was not significantly associated with the need for surgical intervention nor was it significantly associated with the necessity of globe‐sparing surgery versus enucleation. Additionally, bacterial co‐infection, previous steroidal or antifungal use, and time of year did not affect outcome or type of fungal species cultured.ConclusionEquine keratomycosis fromFusariumspp. compared to keratomycosis fromAspergillusspp. is not associated with a different clinical outcome.