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The Plant Journal
Article
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The Plant Journal
Article . 2016 . Peer-reviewed
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Article . 2016
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Inter‐varietal structural variation in grapevine genomes

Authors: CARDONE, MARIA FRANCESCA; D'ADDABBO, PIETRO; Alkan, Can; BERGAMINI, CARLO; CATACCHIO, CLAUDIA RITA; ANACLERIO, FABIO; CHIATANTE, GIORGIA; +5 Authors

Inter‐varietal structural variation in grapevine genomes

Abstract

SummaryGrapevine (Vitis vinifera L.) is one of the world's most important crop plants, which is of large economic value for fruit and wine production. There is much interest in identifying genomic variations and their functional effects on inter‐varietal, phenotypic differences. Using an approach developed for the analysis of human and mammalian genomes, which combines high‐throughput sequencing, array comparative genomic hybridization, fluorescent in situ hybridization and quantitative PCR, we created an inter‐varietal atlas of structural variations and single nucleotide variants (SNVs) for the grapevine genome analyzing four economically and genetically relevant table grapevine varieties. We found 4.8 million SNVs and detected 8% of the grapevine genome to be affected by genomic variations. We identified more than 700 copy number variation (CNV) regions and more than 2000 genes subjected to CNV as potential candidates for phenotypic differences between varieties.

Countries
Italy, Turkey
Keywords

DNA copy number variations, plant genome, Vitis vinifera L, Plant Science, Procedures, Polymerase Chain Reaction, single nucleotide polymorphism, Vitis vinifera L., Table grapes, genetics, Vitis, In Situ Hybridization, In Situ Hybridization, Fluorescence, Mammals, Comparative Genomic Hybridization, High-throughput sequencing, Vitis vinifera l, Genome, Nucleotides, Fluorescence in situ hybridization, copy number variation, high-throughput sequencing, Polymerase chain reaction, Breweries, genomic variation, candidate genes, In situ hybridization, table grape, Genome, Plant, Candidate gene, DNA Copy Number Variations, comparative genomic hybridization, Crops, Fluorescence, Chromosomes, Candidate genes, Genetic, Plant genome, Genetics, procedures, Copy number variations, Candidate genes; Copy number variation; Genomic variation; High-throughput sequencing; Single nucleotide polymorphism; SRP009057; Table grape; Vitis vinifera L.; Genetics; Plant Science; Cell Biology, fluorescence in situ hybridization, Genomic variation, 580, Comparative genomic hybridization, Copy number variation, Table grape, Cell Biology, Plant, Single nucleotide polymorphisms, Throughput, Single nucleotide polymorphism, Genes, SRP009057

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    popularity
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    Top 10%
    influence
    This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
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    impulse
    This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
48
Top 10%
Top 10%
Top 10%
Green
bronze