Abstract Plastome loss is an extremely unusual phenomenon in land plants, even for those that lose their photosynthetic ability. To date, evidence for plastome loss has only been presented for two holoparasitic angiosperm lineages: two genera ( Rafflesia and Sapria ) of Rafflesiaceae and one section ( Subulatae ) of Cuscuta (Convolvulaceae). Here we propose plastome loss in holoparasitic Mystropetalaceae (Santalales), which consists of three monotypic genera: Dactylanthus, Hachettea , and Mystropetalon . Using Illumina DNA sequencing data, we successfully assembled the complete mitochondrial genomes of all three genera; however, we failed to recover any plastome sequences from them using reference-based and de novo assembly approaches. Illumina RNA sequencing data for Hachettea revealed the absence of plastid gene transcripts and losses of many nuclear-encoded genes with plastid function, including genes involved in DNA transcription, translation and protein degradation. Convergent loss of these key genes has also been documented in other plastome-less non-photosynthetic plant lineages. Taken together, we provided compelling evidence for plastome loss in the family Mystropetalaceae.