AbstractBeing shaded is a common environmental stress for plants, especially for densely planted crops. Shade decreases red: far‐red (R:FR) ratios that inactivate phytochrome B (PHYB) and subsequently release p̱hytochrome i̱nteraction f̱actors (PIFs). Shaded plants display elongated hypocotyls, internodes, and petioles, hyponastic leaves, early flowering and are inhibited in branching: traits collectively called the shade avoidance syndrome (SAS). ZEITLUPE (ZTL) is a circadian clock component and blue light photoreceptor, which is also involved in floral rhythms and plant defense inNicotiana attenuata.ztlmutants are hypersensitive to red light and ZTL physically interacts with PHYB, suggesting the involvement of ZTL in R:FR light signaling. Here, we show thatN. attenuataZTL‐silenced plants display a phenotype opposite to that of the SAS under normal light. After simulated shade, the normally induced transcript levels of the SAS marker gene,ATHB2are attenuated in ZTL‐silenced plants. The auxin signaling pathway, known to be involved in SAS, was also significantly attenuated. Furthermore, NaZTL directly interacts with NaPHYBs, and regulates the transcript levels ofPHYBs, PIF3a, PIF7 and PIF8under shade. Our results suggest that ZTL may regulate PHYB‐ and the auxin‐mediated signaling pathway, which functions in the SAS ofN. attenuata.