Bacterium‐Expressed dsRNA Downregulates Microsporidia Nosema bombycis Gene Expression
Copyright policy )Bacterium‐Expressed dsRNA Downregulates Microsporidia Nosema bombycis Gene Expression
AbstractThe microsporidia Nosema bombycis is the insect pathogen of pebrine disease severely destructive to sericulture production. Here, we describe the use of Escherichia coli HT115 strain (DE3) to express double‐strand RNAs targeting the gene encoding ADP/ATP protein in N. bombycis. The results showed that dsRNAs deferentially suppressed the gene expression during N. bombycis infection in the silkworm, and the effect waned gradually. Our results, for the first time, provide a tool to utilize the dsRNA expressed by recombinant E. coli to control the pebrine disease of the domestic silkworm.
- Southeast University China (People's Republic of)
- Chongqing Normal University China (People's Republic of)
- Yunnan Academy of Agricultural Sciences China (People's Republic of)
- Chongqing Normal University China (People's Republic of)
Spores, Down-Regulation, Bombyx, Recombinant Proteins, Animal Diseases, Fungal Proteins, Gene Expression Regulation, Nosema, Microsporidiosis, Escherichia coli, Animals, RNA Interference, Carrier Proteins, DNA, Fungal, RNA, Double-Stranded
Spores, Down-Regulation, Bombyx, Recombinant Proteins, Animal Diseases, Fungal Proteins, Gene Expression Regulation, Nosema, Microsporidiosis, Escherichia coli, Animals, RNA Interference, Carrier Proteins, DNA, Fungal, RNA, Double-Stranded
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