Studies into the microbial spectrum of apical periodontitis
Copyright policy )Studies into the microbial spectrum of apical periodontitis
SummaryThis study examined the variety of obligate and facultative anaerobic bacterial species recovered from cases of acute apical periodontitis. A total of 19 root canal samples and 24 periapical granuloma samples were taken from patients suffering pain and discomfort. Bacteria were identified by applying the following techniques: culturing on various media, Gram‐staining and using commercially available biochemical test strips. In addition, Prevotella intermedia and Porphyromonas endodontalis were differentiated on a molecular genetic level using species‐specific oligodeoxymicleotide probes. The most frequently identified bacteria were Prevotella intermedia, Bifidobacterium spp., Streptococcus sanguis. Streptococcus milleri‐group and Bacteroides spp. Obligate anaerobes occured at a rate of 82.3%, and the average number of isolates was 6.4 per sample.
- Institute of Medical Microbiology and Hygiene Germany
- RWTH Aachen University Germany
Adult, DNA, Bacterial, Male, Gram-Negative Anaerobic Bacteria, Adolescent, Eubacterium, Fusobacterium, Middle Aged, Bacterial Typing Techniques, Actinomyces, Bacteroides, Humans, Female, Bifidobacterium, Dental Pulp Cavity, Child, DNA Probes, Capnocytophaga, Periapical Granuloma, Periapical Periodontitis
Adult, DNA, Bacterial, Male, Gram-Negative Anaerobic Bacteria, Adolescent, Eubacterium, Fusobacterium, Middle Aged, Bacterial Typing Techniques, Actinomyces, Bacteroides, Humans, Female, Bifidobacterium, Dental Pulp Cavity, Child, DNA Probes, Capnocytophaga, Periapical Granuloma, Periapical Periodontitis
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