AbstractThis is the characterisation of the reproductive dynamics of Rhinella schneideri in an urban area during two non‐consecutive periods. Germ cells are similar to those of other anuran species, but some characteristics were more marked for R. schneideri, such as the morphological similarity of spermatogonia and oogonia, which may be related to their potential for development into both stem cell types. The presence of lampbrush chromosomes, the extensive variation in number and organisation of the nucleoli and the organisation of the nuclear cortex are also characteristic of this species. All of these features appear to work together, participating in the dynamic growth of the oocyte. In some specimens, previtellogenic oocytes were also observed in the seminiferous tubules, which may be related to anthropogenic changes induced in the study area. In the first study period, reproductive dynamics revealed synchrony between the sexes, with the preparation of the gonads from October to December and the reproductive season from October to January, coinciding with the rainy season. In the second period, reproductive synchrony was also observed, but gonad preparation occurred from February to June and the reproductive season was from August to November, before the rainy season. These changes in the reproductive cycle suggest that urbanisation affects the reproduction of this species.