AbstractIn order to determine the effect of different oligosaccharides on growth performance, intestinal health, and antioxidant status of broilers, 240 1‐day‐old XiangHuang broilers were randomly distributed to 4 treatments with 6 replicates each. Birds were fed corn‐soybean‐based diets (CON), and birds in xylo‐oligosaccharides (XOS), fructo‐oligosaccharides (FOS), and iso‐maltooligosaccharide (IMO) groups were given the basal diet supplemented with 200 mg/kg XOS, FOS, and IMO, respectively. Result showed that average daily gain (ADG) during the whole 5 weeks in FOS group was greater than that in control group (p < 0.05). Both breast and thigh muscle percentages were higher for birds fed XOS versus CON (p < 0.05). Oligosaccharides supplementation increased jejunal villus height compared with control group (p < 0.05). Malondialdehyde (MDA) concentration in breast muscle was lower for birds fed diet containing FOS versus CON (p < 0.05). Activities of total superoxide dismutase (T‐SOD) in serum and thigh muscle were higher in IMO than in control group (p < 0.05). Serum T‐SOD and breast muscle's glutathione peroxidase (GSH‐Px) activity was higher in XOS compared with control group (p < 0.05). Conclusion, dietary oligosaccharides such as XOS, FOS, and IMO could improve intestinal health and antioxidant ability of muscle without affect growth performance in broilers.