AbstractVarious viral diseases and bacterial soft rot are the major limiting factors for the cultivation of calla lilies (Zantedeschia spp.). Konjac mosaic virus (KoMV) is one of the major causal agents of viral diseases in calla lily fields in Taiwan. The objectives of this study are to identify potential insect vectors of KoMV and to assess the biotic and abiotic factors affecting aphid transmission of KoMV to calla lily. In this study, we identified Myzus persicae and Aphis gossypii as potential vectors of KoMV. Early‐instar nymphs, late‐instar nymphs, apterous adults and alate adults of both aphid species were able to transmit KoMV, and no significant differences in the transmission rates were detected. The difference in KoMV transmission rates was not detected when the transmission assays were performed at 18, 23 and 28°C. Transmission biology is a crucial component of plant disease epidemiology caused by viruses. Vector propensity and vector activity need to be further investigated in the field. The knowledge will be valuable for developing disease control strategies for KoMV in calla lily fields.