In this paper, we have successfully demonstrated the capability of a direct-write piezoelectric poly(vinylidene fluoride) (PVDF) nanogenerator using near-field electrospinning (NFES). Repeated and consistent output voltage up to 8.5 mV has been generated under an external strain of 0.092% from a single electrospun PVDF nanofiber, of which the output power is approximately 7.2 pW. We believe this technology can open up unprecedented possibilities in the field of NEMS/MEMS to convert mechanical strain into electricity for energy harvesting or strain sensing applications.