Marine CSEM (controlled source electromagnetic method) Acquisition Station needs to accurate observe two magnetic field components (Hx, Hy) and three electric field components (Ex, Ey, Ez). In this paper, a series of calibrations are made for the low noise chopper amplifier, which is used in the electric field signal acquisition hardware circuit. The calibration signal generation and the calibration curve calculation process are analyzed in detail. Through the calibration test, the results show that the calibration method is quick and effective. Besides, the amplifier can precisely determine its bandwidth, and verify low noise characteristics.