Mopeds and motorcycles are broadly used and represent a large share of motorized vehicles, above all in urban environment of Asia and Europe. As a result the relative contribution of the emissions of powered two-wheelers to the total air pollution increased considerably in the last years. This paper is aimed at the analysis of the emissions of the new sold motorcycles, contributing widely to extend knowledge of two-wheeler emission behavior, with reference to cold and hot conditions. For this purpose, an experimental investigation was performed: CO, HC and NO X emissions were evaluated in the exhaust of one motorcycle with a displacement of 250 cm3, tested on a dynamometer bench both on a type approval test cycle and on real-world test cycles. This vehicle belongs to the Euro-3 legislative category. The statistical processing results of the experimental data are compared with current emission models for calculating emissions from road traffic in Europe.