In Vitro Fertilization in Mice

descriptionPublicationkeyboard_double_arrow_right Article 18 Apr 2018 English Publisher:Cold Spring Harbor LaboratoryJournal:Cold Spring Harbor Protocols, volume 2,018, page pdb.prot094524 (issn: 1940-3402, eissn: 1559-6095,

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Authors: Toru, Takeo; Naomi, Nakagata;

doi: 10.1101/pdb.prot094524

pmid: 29669849

In Vitro Fertilization in Mice

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Abstract

The success of in vitro fertilization is strongly influenced by genetic background, handling of sperm and oocytes, and culture conditions. This protocol promotes enhanced rates of fertilization by using preincubation medium for sperm containing methyl-β-cyclodextrin (MBCD) and fertilization medium with high calcium and reduced glutathione (GSH).

Keywords

Cryopreservation, Male, Fertilization in Vitro, Embryo Transfer, Spermatozoa, Insemination, Specimen Handling, Embryo Culture Techniques, Mice, Fertilization, Oocytes, Animals, Humans, Female, Cells, Cultured

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