DNA Preparation from Schizosaccharomyces pombe

descriptionPublicationkeyboard_double_arrow_right Article 21 Jul 2017 English Publisher:Cold Spring Harbor LaboratoryJournal:Cold Spring Harbor Protocols, volume 2,018, page pdb.prot091959 (issn: 1940-3402, eissn: 1559-6095,

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Authors: Assen, Roguev; Jiewei, Xu; Nevan J, Krogan;

doi: 10.1101/pdb.prot091959

pmid: 28733411

DNA Preparation from Schizosaccharomyces pombe

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Abstract

This protocol includes two methods for genomic DNA preparation from Schizosaccharomyces pombe. The first is a quick method for preparation of DNA suitable for polymerase chain reaction (PCR) genotyping. The second, longer method yields high-quality DNA that can be used for amplification of targeting cassettes.

Related Organizations

University of California, San Francisco
United States
University of California San Francisco
United States
Department of Cellular and Molecular Pharmacology University of California San Francisco
United States

Keywords

Genetics, Microbial, Genotyping Techniques, Schizosaccharomyces, DNA, Fungal, Mycological Typing Techniques, Molecular Biology, Polymerase Chain Reaction

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