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Nucleic Acids Research
Article . 1992 . Peer-reviewed
Data sources: Crossref
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Targeting deletion (homoeologous chromosome pairing locus) or addition line single copy sequences from cereal genomes

Authors: B, Clarke; P, Stancombe; T, Money; T, Foote; G, Moore;

Targeting deletion (homoeologous chromosome pairing locus) or addition line single copy sequences from cereal genomes

Abstract

We describe here a protocol for obtaining clones containing sequences present in low copy-number from genomic DNA where moderately and highly repeated sequences predominate. Specific chromosomal regions can be targeted by using deletion or addition line material. We have used this protocol to identify a sequence which has been deleted in both the tetraploid and hexaploid wheat mutants for the homoeologous chromosome pairing locus.

Related Organizations
Keywords

Polyploidy, Durapatite, Genome, Mutation, DNA, Hydroxyapatites, Chromosome Deletion, Cloning, Molecular, Triticum

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    influence
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    This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
20
Average
Top 10%
Top 10%
gold