Changes in locomotor activity of beef females during the 72 h prepartum were determined in 3 experiments: (i) a 2-yr study with spring-calving multiparous cows (Exp. 1; n = 34 and 27 for years 1 and 2, respectively); (ii) spring-calving primiparous (first pregnancy; n = 13) and multiparous (n = 21) dams (Exp. 2); and (iii) fall-calving multiparous cows (Exp. 3; n = 33). For all experiments, IceQube activity monitors (iceRobotics, Edinburgh, UK) were placed above the left hind fetlock of pregnant females ≥3 d prepartum. During the calving season, females were housed in 18 × 61 m drylots with ad libitum access to hay or haylage. Parturition was closely monitored, and time of birth was noted. Motion index, standing and lying time, step count, and the number of lying bouts for each dam (summed per hour) were determined for the 72 h preceding calving. Within experiment, data were analyzed by day (days -3 to -1 prepartum), by 6-h period during the final 24 h prepartum, and by hour during the final 6 h prepartum using a mixed model with time as a repeated effect. Year was also included as a fixed effect in Exp. 1. Fixed effects of parity and time prepartum × parity were included for Exp. 2. In all 3 experiments, motion index, standing time, step count, and the number of lying bouts were greater (P < 0.001) on day -1 compared with days -2 and -3 prepartum. In the 24 h prepartum, dams had greater (P < 0.01) motion index, standing time, step count, and the number of lying bouts during 6 h preceding parturition compared with -11 to -6 h in all experiments. Motion index, step count, and lying bouts changed (P ≤ 0.02) during the last 6 h in all experiments. Primiparous dams had more (P ≤ 0.01) lying bouts than multiparous dams during the last day and -11 to -6 prepartum. In all experiments, the number of lying bouts more than doubled (P < 0.001) from -2 to -1 prepartum, with no effect of year (P = 0.57) in Exp. 1 or parity (P ≥ 0.29) in Exp. 2. This suggests that lying bout changes may be the most reliable of parameters measured in detection of calving. Moreover, fall-calving cow behavioral patterns were similar to changes observed in spring-calving females, suggesting that calving season may have minimal effects on pre-calving behavior. Overall, electronic locomotor activity monitors can detect behavioral changes peripartum in beef heifers and cows. More research is necessary to determine if these can be used to remotely sense early signs of parturition in beef cattle.