Abstract Within the rich spectrum of musical documents from medieval Bohemia, the corpus of tropes that survives in manuscripts from St George’s convent at Prague Castle is perhaps the most important. Forty-two Benedicamus Domino tropes appear in manuscripts from the monastery—one of the richest and most important female foundations in Central Europe, with a small community of nuns from noble families—most of them compiled at the end of the 13th and the first decades of the 14th centuries. The collection includes Benedicamus tropes that were circulating in the wider Central European territory, tropes that had their origins in the west and for which Prague constituted one of the furthest destinations within their dissemination, and a significant number of chants survive only in manuscripts from St George’s and may have had their origins in this milieu. These Benedicamus tropes exhibit a striking variety of forms and richness in their melodic material: a number of polyphonic tropes employ a Stimmtausch technique, others are wholly structured as strophic songs with or without refrains. Many of the trope texts indicate that they were meant to be sung by both male and female communities at St George’s, and their appearance in books with private prayers suggests that they may also have served an educational purpose.