Abstract Internet network needs are increasingly rapid in supporting current business needs. This goes hand in hand with requests for IP Address allocations for companies to measure information and business needs. The increasing demand for current IP Address allocation, the fewer the number of IP Addresses available. To fulfill the demand from the use of IP Address, Internet Protocol version 6 (IPv6) or internet protocol was adopted as the next-generation Internet technology. Exchange of IP Address in computer network cannot be separated from the existence of routing protocol. Enhanced Interior Gateway Routing Protocol (EIGRP) also developed with the use of IPv6 to EIGRP for IPv6 (EIGRPv6). The EIGRPv6 routing concept is designed so well that it is capable of handling table routing that is very large and more efficient than the previous EIGRP. In large-scale computer networks must use good security. One method of network security is the Access Control List (ACL) capable of supporting the performance of IPv6. The application of ACL in this study is able to filter the access of network users by using IPv6 Extended ACL. Based on the results of EIGRPv6 network testing, the average packet delivery time is 7.6ms. And the application of IPv6 ACL is able to limit access to Web Servers, ICMP and FTP Servers properly.