Polyspora axillaris (Roxb. ex Ker Gawl.) Sweet 1825, is a shrub or tree that is about 9 meters tall in the Theaceae family, mainly distributed in China and Vietnam, and it is widely used as a green tree species in many regions owing to its rapid growth and good adaptability. It is rich in various beneficial extracts for humans, but there are limited studies on it. In this study, we sequenced and annotated the complete plastome of P. axillaris. The chloroplast genome length of P. axillaris is 156,770 bp, with a total of 132 genes, including 37 tRNA genes, 8 rRNA genes and 87 protein-coding genes. The complete chloroplast genome of P. axillaris contains two Inverted Repeats (IRs) of 26,077 bp, a Large Single-Copy (LSC) region of 86,286 bp and a Small Single-Copy (SSC) region of 18,330 bp. The overall G/C content in the chloroplast is 37.3%. Phylogenetic inference shows that P. axillaris formed a sister relationship with P. hainanensis, along with 10 Theaceae species. The research result of P. axillaris will contribute to the genetic preservation of the species and the phylogenetic study of Polyspora.