The procedure of Stanford and coworkers was used to quantitatively relate net nitrogen mineralization in five Queensland semi-arid soils to temperature. The concentration of potentially mineralizable nitrogen (No) (1) ranged from 67 µg nitrogen g-1 for a red earth subsoil to 256 for a recently cultivated cracking clay surface soil, (2) was directly proportional to total soil carbon, (3) was greater in surface than in subsurface soil, and (4) was greater in subtropical than tropical soils. Expressed as a fraction of total nitrogen (No/Total N), it ranged between 8 and 21%, and was directly proportional to cation exchange capacity, perhaps implicating expanding lattice clays in stabilization of cell lysates and metabolites. The mineralization rate constant (k) was directly proportional to total carbon, the fuel for microbially mediated reactions in soil. The average k for surface soils was interpolated to be 0.058, 0.031, and 0.018 week-1, corresponding to half-lives of 11.9, 22.4 and 38.5 weeks, at 35°, 25° and 15°C, respectively; these values are similar to those reported for U.S.A. and Chilean soils. The Arrhenius relationship between k and temperature for surface soils (log k = 6.14-2285/T) was similar to that reported by Stanford for U.S.A. soils, and indicates that this relationship might be a general one.