Nanofluidics is generally described as the study of liquid flow in or around structures of 100 nm or smaller, and its use for lab on a chip devices has now been actively studied for two decades. Here a brief review is given of the impact that this nanofluidics research has had on point of care applications. Four areas are identified where nanofluidics has brought the largest contributions: single nanopores, nanoporous membranes, nanoconfinement and the use of concentration polarization. The sometimes revolutionary developments in these areas are briefly treated and finally challenges and future perspectives are described.