Protected DNA strand displacement for enhanced single nucleotide discrimination in double-stranded DNA
Copyright policy )Protected DNA strand displacement for enhanced single nucleotide discrimination in double-stranded DNA
Single nucleotide polymorphisms (SNPs) are a prime source of genetic diversity. Discriminating between different SNPs provides an enormous leap towards the better understanding of the uniqueness of biological systems. Here we report on a new approach for SNP discrimination using toehold-mediated DNA strand displacement. The distinctiveness of the approach is based on the combination of both 3- and 4-way branch migration mechanisms, which allows for reliable discrimination of SNPs within double-stranded DNA generated from real-life human mitochondrial DNA samples. Aside from the potential diagnostic value, the current study represents an additional way to control the strand displacement reaction rate without altering other reaction parameters and provides new insights into the influence of single nucleotide substitutions on 3- and 4-way branch migration efficiency and kinetics.
- Flinders University Australia
- University of Melbourne Australia
- University of Adelaide Australia
570, Genotyping Techniques, DNA Mutational Analysis, Molecular Sequence Data, 612, Nucleic Acid Denaturation, DNA, Mitochondrial, Polymerase Chain Reaction, Polymorphism, Single Nucleotide, Article, Genetic, Models, Humans, Transition Temperature, Polymorphism, Base Sequence, Models, Genetic, Nucleotides, Nucleic Acid Hybridization, Reproducibility of Results, Nanobiotechnology, Single Nucleotide, DNA, Mitochondrial, Kinetics
570, Genotyping Techniques, DNA Mutational Analysis, Molecular Sequence Data, 612, Nucleic Acid Denaturation, DNA, Mitochondrial, Polymerase Chain Reaction, Polymorphism, Single Nucleotide, Article, Genetic, Models, Humans, Transition Temperature, Polymorphism, Base Sequence, Models, Genetic, Nucleotides, Nucleic Acid Hybridization, Reproducibility of Results, Nanobiotechnology, Single Nucleotide, DNA, Mitochondrial, Kinetics
selected citations These citations are derived from selected sources.This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).26 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Top 10% influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Top 10% impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Top 10%
Citations provided by BIP!Popularity provided by BIP!