Thermal proteome profiling monitors ligand interactions with cellular membrane proteins
Copyright policy )Thermal proteome profiling monitors ligand interactions with cellular membrane proteins
We extended thermal proteome profiling to detect transmembrane protein-small molecule interactions in cultured human cells. When we assessed the effects of detergents on ATP-binding profiles, we observed shifts in denaturation temperature for ATP-binding transmembrane proteins. We also observed cellular thermal shifts in pervanadate-induced T cell-receptor signaling, delineating the membrane target CD45 and components of the downstream pathway, and with drugs affecting the transmembrane transporters ATP1A1 and MDR1.
Proteomics, ATP Binding Cassette Transporter, Subfamily B, Hot Temperature, Proteome, Protein Stability, Receptors, Antigen, T-Cell, Membrane Proteins, Ligands, Small Molecule Libraries, Jurkat Cells, Tandem Mass Spectrometry, Humans, Caco-2 Cells, Sodium-Potassium-Exchanging ATPase, Vanadates, K562 Cells, Protein Binding
Proteomics, ATP Binding Cassette Transporter, Subfamily B, Hot Temperature, Proteome, Protein Stability, Receptors, Antigen, T-Cell, Membrane Proteins, Ligands, Small Molecule Libraries, Jurkat Cells, Tandem Mass Spectrometry, Humans, Caco-2 Cells, Sodium-Potassium-Exchanging ATPase, Vanadates, K562 Cells, Protein Binding
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