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Biophysical Journal
Article . 2015
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Nature Methods
Article . 2015
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Acoustic force spectroscopy

Authors: Gerrit Sitters; Douwe Kamsma; Gregor Thalhammer; Monika Ritsch-Marte; Erwin J G Peterman; Gijs J L Wuite;

Acoustic force spectroscopy

Abstract

Force spectroscopy has become an indispensable tool to unravel the structural and mechanochemical properties of biomolecules. Here we extend the force spectroscopy toolbox with an acoustic manipulation device that can exert forces from subpiconewtons to hundreds of piconewtons on thousands of biomolecules in parallel, with submillisecond response time and inherent stability. This method can be readily integrated in lab-on-a-chip devices, allowing for cost-effective and massively parallel applications.

Country
Netherlands
Keywords

Spectrum Analysis, Microfluidics, Biophysics, Acoustics, DNA, Equipment Design, Biophysical Phenomena, Microspheres, Antigen-Antibody Reactions, DNA-Binding Proteins, Rec A Recombinases, Lab-On-A-Chip Devices, SDG 7 - Affordable and Clean Energy, Digoxigenin, Molecular Biology

  • BIP!
    Impact byBIP!
    selected citations
    These citations are derived from selected sources.
    This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
    192
    popularity
    This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
    Top 1%
    influence
    This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
    Top 10%
    impulse
    This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
    Top 1%
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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
192
Top 1%
Top 10%
Top 1%
hybrid