Correcting CRISPR for copy number

descriptionPublicationkeyboard_double_arrow_right Article 29 Nov 2017 English Publisher:Springer Science and Business Media LLCJournal:Nature Genetics, volume 49, pages 1,674-1,675 (issn: 1061-4036, eissn: 1546-1718,

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Authors: John Paul, Shen; Trey, Ideker;

doi: 10.1038/ng.3994

pmid: 29186130

Correcting CRISPR for copy number

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Abstract

The CRISPR-Cas9 system enables global screens of gene function with high sensitivity and specificity, but off-target effects have been reported for CRISPR guide RNAs targeting genes that are amplified at high copy number. A new study describes a computational approach to correct for this copy number effect, increasing the specificity of CRIPSR screens to identify essential genes.

Related Organizations

University of California, San Diego
United States

Keywords

Genes, Essential, DNA Copy Number Variations, Clustered Regularly Interspaced Short Palindromic Repeats, CRISPR-Cas Systems, RNA, Guide, CRISPR-Cas Systems, Sensitivity and Specificity

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