Lipidic cubic phase injector facilitates membrane protein serial femtosecond crystallography
Copyright policy )Lipidic cubic phase injector facilitates membrane protein serial femtosecond crystallography
Lipidic cubic phase (LCP) crystallization has proven successful for high-resolution structure determination of challenging membrane proteins. Here we present a technique for extruding gel-like LCP with embedded membrane protein microcrystals, providing a continuously renewed source of material for serial femtosecond crystallography. Data collected from sub-10-μm-sized crystals produced with less than 0.5 mg of purified protein yield structural insights regarding cyclopamine binding to the Smoothened receptor.
- Autonomous University of Madrid Spain
- Universität Hamburg Germany
- Trinity College Dublin Ireland
- Max Planck Society Germany
- Arizona State University United States
Lipidic cubic phase (LCP), 570, Crystallography, Membrane Proteins, 540, Lipids, info:eu-repo/classification/ddc/500, Receptors, G-Protein-Coupled
Lipidic cubic phase (LCP), 570, Crystallography, Membrane Proteins, 540, Lipids, info:eu-repo/classification/ddc/500, Receptors, G-Protein-Coupled
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