A RECEPTOR ASSAY FOR SOMATOMEDIN‐LIKE ACTIVITY USING MULTIPLICATION‐STIMULATING ACTIVITY (MSA) AS RADIOLIGAND
Copyright policy )A RECEPTOR ASSAY FOR SOMATOMEDIN‐LIKE ACTIVITY USING MULTIPLICATION‐STIMULATING ACTIVITY (MSA) AS RADIOLIGAND
SummaryMultiplication‐stimulating activity (MSA) in the medium of cultured rat liver cells was purified 120‐fold by chromatography on Dowex 50W and Sephadex G 50. MSA was labelled with 125I‐iodine and bound to a human placental membrane receptor. An assay for somatomedin‐like receptor activity (SmLRA) in unextracted serum was based on the displacement of 125I‐MSA from the placental receptor. The mean λ (precision) was 0·063, sensitivity was < 0·006 SmLRA units and interassay variation was 18%. The mean (± S.D.) SmLRA concentrations in 13 normal subjects, 7 growth hormone deficient patients and 8 patients with active acromegaly were 0·86 ± 0·19, 0·34 ± 0·08 and 2·34 ± 0·82 U/ml, respectively.This assay may have potential clinical application in disorders of growth hormone secretion.
- University of Queensland Australia
- University of Auckland New Zealand
- Royal Brisbane and Women's Hospital Australia
Adult, Male, Adolescent, Placenta, Fibroblasts, Receptor, Insulin, Rats, Radioligand Assay, Cartilage, Liver, Pregnancy, Growth Hormone, Acromegaly, Animals, Humans, Female, Child, Peptides, Hypophysectomy, Protein Binding
Adult, Male, Adolescent, Placenta, Fibroblasts, Receptor, Insulin, Rats, Radioligand Assay, Cartilage, Liver, Pregnancy, Growth Hormone, Acromegaly, Animals, Humans, Female, Child, Peptides, Hypophysectomy, Protein Binding
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