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Single Molecule Observation of DNA Electrophoresis in Pluronic F127

Authors: Seungyong, You; David H, Van Winkle;

Single Molecule Observation of DNA Electrophoresis in Pluronic F127

Abstract

Single molecule fluorescence microscopy is used to follow the motion of long DNA molecules undergoing electrophoresis in Pluronic gels. We find that for low fields most DNA molecules follow tortuous paths through the gels, at an angle up to 90 degrees from the field direction, while some molecules find paths along the field lines. In high fields, virtually all of the DNA molecules follow the field lines. In many cases, the molecules travel as compact coils with optically discernible radii smaller than in free solution. In other cases, the molecules extend and contract or travel in an extended configuration.

Related Organizations
Keywords

Electrophoresis, Microscopy, Fluorescence, DNA, Poloxamer

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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
10
Average
Average
Average
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