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Ceramide Kinase and Ceramide‐1‐Phosphate

descriptionPublicationkeyboard_double_arrow_right Part of book or chapter of book , Article 01 Jan 2007Publisher:Elsevier BV

Authors: Antonio Gómez-Muñoz; Nadia F. Lamour; Charles E. Chalfant; Charles E. Chalfant; Dayanjan S. Wijesinghe;

doi: 10.1016/s0076-6879(07)34015-9

pmid: 17954253

Ceramide Kinase and Ceramide‐1‐Phosphate

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Abstract

It has been over a decade since the sphingolipid ceramide-1-phosphate (C1P) was described. Until recently, only sparse reports on possible biological functions for this lipid have been published. A large number of reports have now surfaced demonstrating distinct biological mechanisms regulated by C1P produced from ceramide kinase (CERK). In the following methods chapter, the methodologies for examining CERK function in vitro and in cells are outlined in detail. The methodologies for examining C1P levels and the use of exogenous C1P on cells to observe lipid specific effects on a particular biology are also detailed.

Related Organizations

United States Department of the Interior
United States
University of the Basque Country
Spain
Virginia Commonwealth University
United States
Hunter Holmes McGuire VA Medical Center
United States

Keywords

Diacylglycerol Kinase, Molecular Sequence Data, Spodoptera, Ceramides, Transfection, Recombinant Proteins, Kinetics, Phosphotransferases (Alcohol Group Acceptor), Catalytic Domain, Animals, Amino Acid Sequence

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	citations This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).	30
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	impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.	Top 10%