descriptionPublicationkeyboard_double_arrow_right Article 01 Dec 2002 English Publisher:Elsevier BVJournal:Chemistry and Physics of Lipids, volume 120, pages 75-85 (issn: 0009-3084,

Authors: Michael, Hodenius; Marcel, De Cuyper; Linda, Desender; Detlef, Müller-Schulte; Alois, Steigel; Heiko, Lueken;

doi: 10.1016/s0009-3084(02)00105-6

pmid: 12426077

Biotinylated Stealth® magnetoliposomes

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Abstract

Dimyristoylphosphatidylethanolamine (DC(14:0)PE) and the dioleoyl analogue (DC(18:1cis)PE) were mixed with alpha-biotinylamido-omega-N-succinimidoxycarbonyl-poly(ethylene glycol) (NHS-PEG-biotin) and quantitatively converted to alpha-biotinylamido-omega-(dimyristoylphosphatidylethanolamino-carbonyl)polyethylene glycol (DC(14:0)PE-PEG-biotin) and the dioleoyl analogue DC(18:1cis)PE-PEG-biotin, respectively. As shown by thin-layer chromatography and 1H NMR spectroscopy, PEGylation of both phosphatidylethanolamine types went to completion if the reaction was performed in organic solvent in the presence of triethylamine. The resulting derivatives were successfully incorporated into both classical phospholipid vesicles and a phospholipid bilayer surrounding nanometer-sized magnetite cores. In the latter case, the so-called activated Stealth(1) magnetoliposomes were produced which very efficiently immobilized streptavidinylated alkaline phosphatase.

Related Organizations

RWTH Aachen University
Germany
KU Leuven
Belgium
Heinrich Heine University Düsseldorf
Germany
University of Duesseldorf
Germany
Katholieke Universiteit Leuven
Belgium

Keywords

Drug Delivery Systems, Iron, Liposomes, Biotin, Oxides, Streptavidin, Alkaline Phosphatase, Ferrosoferric Oxide, Polyethylene Glycols

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