Gene targeting in rats using transcription activator-like effector nucleases
Copyright policy )Gene targeting in rats using transcription activator-like effector nucleases
The rat is a model of choice to understanding gene function and modeling human diseases. Since recent years, successful engineering technologies using gene-specific nucleases have been developed to gene edit the genome of different species, including the rat. This development has become important for the creation of new rat animals models of human diseases, analyze the role of genes and express recombinant proteins. Transcription activator-like (TALE) nucleases are designed nucleases consist of a DNA binding domain fused to a nuclease domain capable of cleaving the targeted DNA. We describe a detailed protocol for generating knockout rats via microinjection of TALE nucleases into fertilized eggs. This technology is an efficient, cost- and time-effective method for creating new rat models.
570, DNA End-Joining Repair, Microinjections, Knockout, 610, Gene editing, Rats, Sprague-Dawley, Gene Knockout Techniques, Animals, Homologous recombination, Homologous Recombination, [SDV.MHEP] Life Sciences [q-bio]/Human health and pathology, Deoxyribonucleases, TALE nucleases, Embryo Transfer, Embryo, Mammalian, Rats, Rat model, Mutagenesis, Site-Directed, Female, Non-homologous end joining, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology
570, DNA End-Joining Repair, Microinjections, Knockout, 610, Gene editing, Rats, Sprague-Dawley, Gene Knockout Techniques, Animals, Homologous recombination, Homologous Recombination, [SDV.MHEP] Life Sciences [q-bio]/Human health and pathology, Deoxyribonucleases, TALE nucleases, Embryo Transfer, Embryo, Mammalian, Rats, Rat model, Mutagenesis, Site-Directed, Female, Non-homologous end joining, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology
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