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Trends in Microbiology
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SARS-CoV-2 viral RNA levels are not 'viral load'

Authors: Michalakis, Yannis; Sofonea, Mircea; Alizon, Samuel; Bravo, Ignacio G.;

SARS-CoV-2 viral RNA levels are not 'viral load'

Abstract

Ct values are commonly used as proxy of SARS-CoV-2 “viral load”. Because of the (+)ssRNA nature of coronaviruses, current RT-qPCR target amplification does not distinguish replicative from transcriptional RNA. Although Ct values analyses through time, space and biological backgrounds remain informative, equating Ct with viral load may generate misleading conclusions.

Keywords

[SDV.MHEP.ME] Life Sciences [q-bio]/Human health and pathology/Emerging diseases, [SDV.MP.VIR] Life Sciences [q-bio]/Microbiology and Parasitology/Virology, Transcription, Genetic, Forum, SARS-CoV-2, RT-qPCR, Life Sciences, COVID-19, Viral Load, Real-Time Polymerase Chain Reaction, Virus Replication, Microbiology, viral load, COVID-19 Testing, Virology, [SDV.MHEP.MI] Life Sciences [q-bio]/Human health and pathology/Infectious diseases, viral replication, Humans, RNA, Viral, viral transcription, Immunology and Infectious Disease, Ct

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    selected citations
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    This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
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    popularity
    This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
    Top 10%
    influence
    This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
    Top 10%
    impulse
    This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
28
Top 10%
Top 10%
Top 10%
Green
hybrid