Abstract Autoignition-assisted nonpremixed cool flames of diethyl ether (DEE) are investigated in both laminar counterflow and turbulent jet flame configurations. First, the ignition and extinction limits of laminar nonpremixed cool flames of diluted DEE are measured and simulated using detailed kinetic models. The laminar flame measurements are used to validate the kinetic models and guide the turbulent flame measurements. The results show that, below a critical mixture condition, for elevated temperature and dilute mixtures, the cool flame extinction limit and the low-temperature ignition limit merge, leading to autoignition-assisted cool flame stabilization without hysteresis. Based on the findings from the laminar flame experiments, autoignition-assisted turbulent lifted cool flames are established using a Co-flow Axisymmetric Reactor-Assisted Turbulent (CARAT) burner. The lift-off heights of the turbulent cool flames are quantified using formaldehyde planar laser-induced fluorescence. Based on an analogy with autoignition-assisted lifted hot flames, a correlation is proposed such that the autoignition-assisted cool flame lift-off height scales with the product of the flow velocity and the square of the first-stage ignition delay time. Using this scaling, we demonstrate that the kinetic mechanism that most accurately predicts the laminar flame ignition and extinction limits also best predicts the turbulent cool flame lift-off height.