PCR amplification of species specific sequences of 16S rDNA and 16S–23S rDNA intergenic spacer region for identification of Streptococcus phocae
Copyright policy )PCR amplification of species specific sequences of 16S rDNA and 16S–23S rDNA intergenic spacer region for identification of Streptococcus phocae
Streptococcus phocae, a bacterial pathogen of seals, could reliably be identified by PCR amplification using oligonucleotide primers designed according to species specific segments of the previously sequenced 16S rRNA gene and the 16S-23S rDNA intergenic spacer region of this species. The PCR mediated assay allowed an identification of S. phocae isolated from harbor and gray seals and from Atlantic salmons. No cross-reaction could be observed with 13 different other streptococcal species and subspecies and with Lactococcus garvieae strains investigated for control purposes.
DNA, Bacterial, Seals, Earless, Molecular Sequence Data, Salmo salar, Streptococcus, Sequence Analysis, DNA, Microbiology, DNA, Ribosomal, Polymerase Chain Reaction, Bacterial Typing Techniques, Fish Diseases, RNA, Ribosomal, 23S, Species Specificity, RNA, Ribosomal, 16S, Streptococcal Infections, DNA, Ribosomal Spacer, Animals
DNA, Bacterial, Seals, Earless, Molecular Sequence Data, Salmo salar, Streptococcus, Sequence Analysis, DNA, Microbiology, DNA, Ribosomal, Polymerase Chain Reaction, Bacterial Typing Techniques, Fish Diseases, RNA, Ribosomal, 23S, Species Specificity, RNA, Ribosomal, 16S, Streptococcal Infections, DNA, Ribosomal Spacer, Animals
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