The seminal work \cite{bm} by Brezis and Merle showed that the bubbling solutions of the mean field equation have the property of mass concentration. Recently, Lin and Tarantello in \cite{lt} found that the "bubbling implies mass concentration" phenomena might not hold if there is a collapse of singularities. Furthermore, a sharp estimate \cite{llty} for the bubbling solutions has been obtained. In this paper, we prove that there exists at most one sequence of bubbling solutions if the collapsing singularity occurs. The main difficulty comes from that after re-scaling, the difference of two solutions locally converges to an element in the kernel space of the linearized operator. It is well-known that the kernel space is three dimensional. So the main technical ingredient of the proof is to show that the limit after re-scaling is orthogonal to the kernel space.