The ecological environment of the Gurbantünggüt desert-oasis ecotone is extremely fragile. Ephemeral plants are an important part of the ecosystem and play an essential role in maintaining the ecological stability of the ecotone. However, few studies have focused on the growth, soil quality and system sustainability of ephemeral plants in different soils. This study was based on two typical soil types (grey desert soil, GS; aeolian soil, AS) in the aforementioned ecotone, considered four ephemeral plants (Tetracme recurvata, TR; Tetracme contorta, TC; Malcolmia scorpioides, MS; Isatis violascens, IV) as the research object, analysed plant characteristics and soil properties, and comprehensively evaluated the ephemeral plant system by analysing the soil quality index (SQI) and sustainability index (SI). The results showed that there were significant differences in biomass and nutrient accumulation between different ephemeral plants, which were significantly affected by soil types. In the two examined soils (GS and AS), the contents of nutrients and microbial carbon (MBC) and nitrogen (MBN) in the rhizosphere soil were higher than those in the bare soil (BS), and there were significant differences among different species. The key soil factors related to total biomass in GS and AS were also different. The SQI of ephemeral plants was significantly higher than that of the BS, and varied with soil types and plant species. The species with the highest SQI of the key factor data set in GS and AS were IV and TR, respectively. The SI analysis indicated that IV in GS and MS and IV in AS were sustainable, and the plant properties can be better used to assess the sustainability of ephemeral plant systems. In conclusion, ephemeral plants improved the soil quality and system sustainability of the study ecotone. Further, the growth of ephemeral plant and rhizosphere soil properties vary with plant species and soil types; thus, selecting suitable species for large-scale planting in different soil types is of great significance for improving the ecological stability of the ecotone.