The effect of starch-protein interactions on the ability of linear starch chains to bind iodine was investigated in 4 types of semolina. Based on K/S (absorption/scattering coefficient) spectra, obtained after equilibration above K2SO4 and exposure to iodine vapor, and X-ray diffraction, semolina samples showed differences in chain mobility, iodine-binding capacity and crystalline order. After removing protein from the samples, starch exhibited a higher iodine-binding capacity, suggesting greater starch chain mobility, and low crystalline order. The results suggest that protein and/or starch-protein affect the packing arrangement of starch polymers within the granule.