Large scale green tides have bloomed successively in the Yellow Sea since 2007. The floating ecotype of Ulva prolifera, which is responsible for the environmental disaster, drifted a long distance during the blooming time and was exotic to the coastal area. The Yellow Sea green tide can be a potential source to incur bio-invasion. In this study, the distribution pattern and propagule pressure of the floating ecotype was investigated along the Qingdao coastline, which was seriously impacted by the green tide. Two out of 661 attached Ulva specimens collected in different seasons were identified as the floating ecotype by molecular markers, indicating that a few individuals of the floating ecotype had settled down, and their attached population could have spontaneously established. In seawater and sediments, the proportion of the floating ecotype in Ulva propagules reached up to 32% and 69% respectively when the floating algae was accumulating on seashore, which was a great propagule pressure to the local ecosystem. Results of the field test indicated that the available resources and the competition between the floating ecotype and the local Ulva species might be the main restrictions for settlement. Though the current scale of the established population is still small, the risk of biological invasion by the floating ecotype exists and it deserves more attention.