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Food Microbiology
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Genetic analysis of geraniol metabolism during fermentation

Authors: Steyer, Damien; Erny, Claude; Claudel, Patricia; Riveill, Genevieve; Karst, Francis; Legras, Jean-Luc;

Genetic analysis of geraniol metabolism during fermentation

Abstract

Geraniol produced by grape is the main precursor of terpenols which play a key role in the floral aroma of white wines. We investigated the fate of geraniol during wine fermentation by Saccharomyces cerevisiae. The volatile compounds produced during fermentation of a medium enriched with geraniol were extracted by Stir-bar sorptive extraction and analysed by GC-MS. We were able to detect and quantify geranyl acetate but also citronellyl- and neryl-acetate. The presence of these compounds partly explains the disparition of geraniol. The amounts of terpenyl esters are strain dependant. We demonstrated both by gene overexpression and gene-deletion the involvement of ATF1 enzyme but not ATF2 in the acetylation of terpenols. The affinity of ATF1 enzyme for several terpenols and for isoamyl alcohol was compared. We also demonstrated that OYE2 is the enzyme involved in geraniol to citronellol reduction. Fermenting strain deleted from OYE2 gene produces far less citronellol than wild type strain. Moreover lab strain over-expressing OYE2 allows 87% geraniol to citronellol reduction in bioconversion experiment compared to about 50% conversion with control strain.

Country
France
Keywords

Ethics, 570, Geraniol, Saccharomyces cerevisiae Proteins, Terpenes, Acyclic Monoterpenes, Geranyl acetate, Wine, Saccharomyces cerevisiae, 540, [SDV.ETH] Life Sciences [q-bio]/Ethics, fermentation;citronellol;Saccharomyces cerevisiae; Fermentation;Geraniol;Geranyl acetate;OYE2;ATF1, [SDV.ETH]Life Sciences [q-bio]/Ethics, citronellol, Ethique, Fermentation, OYE2, ATF1, Monoterpenes, fermentation, Gene Deletion

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    popularity
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    Top 10%
    influence
    This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
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    impulse
    This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
61
Top 10%
Top 10%
Top 10%
Green
bronze