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FEMS Microbiology Letters
Article
Data sources: UnpayWall
FEMS Microbiology Letters
Article . 2004 . Peer-reviewed
Data sources: Crossref
FEMS Microbiology Letters
Article . 2005
Data sources: Europe PubMed Central
https://dx.doi.org/10.1016/j.f...
Article
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Quinate oxidation inGluconobacter oxydansIFO3244: purification and characterization of quinoprotein quinate dehydrogenase

descriptionPublicationkeyboard_double_arrow_right Article 01 Dec 2004 English Publisher:Oxford University Press (OUP)Journal:FEMS Microbiology Letters, volume 241, pages 157-162 (issn: 0378-1097, eissn: 1574-6968, Copyright policy )
Authors: Alisa S, Vangnai; Hirohide, Toyama; Wanchai, De-Eknamkul; Nozomi, Yoshihara; Osao, Adachi; Kazunobu, Matsushita;

doi: 10.1016/j.femsle.2004.10.014

pmid: 15598527

Quinate oxidation inGluconobacter oxydansIFO3244: purification and characterization of quinoprotein quinate dehydrogenase

Abstract

Quinoprotein quinate dehydrogenase (QDH) is a membrane-bound enzyme containing pyrroloquinoline quinone (PQQ) as the prosthetic group. QDH in Gluconobacter oxydans IFO3244 was found to be inducible by quinate and it is not constitutively expressed in the absence of quinate. The purification of holo-form of QDH to nearly homogeneity was achieved. The purified QDH appears to have two subunits of approximately 65 and 21 kDa, which could be the result of proteolysis of single polypeptide. Kinetic analysis indicated that the purified enzyme is much more specific to quinate than QDH from Acinetobacter calcoaceticus. The efficiency of the artificial electron acceptor was also determined.

Related Organizations
Keywords

Gluconobacter oxydans, Alcohol Oxidoreductases, Molecular Sequence Data, PQQ Cofactor, Quinic Acid, Amino Acid Sequence, Oxidation-Reduction, Substrate Specificity

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    popularity
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    influence
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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
17
Average
Top 10%
Average
bronze