Lead is toxic to fish, and its toxicity can be aggravated by the water temperature. Geophagus brasiliensis populations are geographically widespread and thus live in areas with different temperatures. The objective of this work was to evaluate the effects of lead-contaminated feed in fish (Geophagus brasiliensis) exposed to different temperatures. A factorial experiment was performed with two temperatures (25 and 28°C), and two feeds (control and lead contaminated - 60 mg/kg) for a sum of four treatments (25°C, Control = 25/C; 25°C, 60 mg/kg = 25/60; 28°C, Control = 28/C and 28°C, 60 mg/kg = 28/60). Analyses of the lead accumulation, oxidative stress and genotoxic damage were performed. The gills and liver showed increased lead concentrations in fish receiving lead-contaminated food at both temperatures. The lead concentrations in the intestines and muscles of fish exposed to the 25/60 treatment was greater than it was in fish exposed to the 25/C treatment. The enzyme response in the gills and the micronuclei count increased in fish exposed to the 25/60 treatment. Higher temperatures can be a beneficial factor for Geophagus brasiliensis because they can hinder the absorption of lead, thereby reducing the damage caused to the organism.