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Egyptian Journal of Forensic Sciences
Article . 2015 . Peer-reviewed
License: CC BY NC ND
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Egyptian Journal of Forensic Sciences
Article
License: CC BY NC ND
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Egyptian Journal of Forensic Sciences
Article . 2015
License: CC BY NC ND
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Evaluation of techniques for human bone decalcification and amplification using sixteen STR markers

Authors: Ajay Balayan; Abhilasha Kapoor; Garima Chaudhary; Anupuma Raina;

Evaluation of techniques for human bone decalcification and amplification using sixteen STR markers

Abstract

AbstractEfficient DNA extraction procedures, as well as accurate DNA amplification, are critical steps involved in the process of successful DNA analysis of skeletal samples. Unfortunately, at present there is no infallible method to recover DNA from highly degraded samples due to variations in DNA yield from larger bone fragments, which may be attributed to heterogeneity within bones. We evaluated two different protocols for bone decalcification in the DNA extraction procedure for bones. This study is important for analysis of challenging forensic samples.

Keywords

Medicine (General), Genotyping, Law in general. Comparative and uniform law. Jurisprudence, R5-920, Short tandem repeats, Decalcification, K1-7720, Multiplex PCR, Sternum bone

  • BIP!
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    selected citations
    These citations are derived from selected sources.
    This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
    6
    popularity
    This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
    Top 10%
    influence
    This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
    Average
    impulse
    This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
    Average
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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
6
Top 10%
Average
Average
gold