Abstract African leafy vegetables (ALVs) play a crucial role in substituting the diets and household income of local people. Production of ALVs is, however, hampered by plant-parasitic nematodes, particularly Meloidogyne spp. The objectives of this study were: i) to determine the host suitability of 13 Amaranthus genotypes to Meloidogyne incognita and M. javanica in separate greenhouse experiments, and at three on-farm field sites, and ii) to verify resistance exhibited by genotype Thohoyandou, identified in greenhouse screenings, to the two said root-knot nematode species in micro plot studies. Glasshouse screenings for M. incognita showed reproduction factor (Rf) values