Abstract Warburgia ugandensis Sprague is an endemic plant to Africa and widely distributed in lower rain forests and drier highland forests of Kenya, Uganda and Ethiopia. In south eastern part of Ethiopia (Bale) W. ugandensis is known as Befti and widely used as traditional medicine for the treatment of various diseases such as asthma, cough, diarrhea, common cold, stomachache and toothache. It is also used to remove tapeworm. Phytochemical investigation on the n-hexane and dichloromethane extracts of the stem bark of this plant afforded two sesquiterpenes; namely, HP-66 (Nerolidol) and HP-79 (Muzigadial). The structural elucidations of these compounds were accomplished by using a variety of spectroscopic methods (IR, UV and NMR). The spectroscopic results compared with the reported data in the literature. HP-66 (Nerolidol) was reported from Warburgia ugandensis Sprague for the first time. Parameters drug-likeness and physicochemical properties including pharmacokinetics analysis of the HP-66 (Nerolidol) and HP-79 (Muzigadial) compounds was performed using online tool Swiss ADME.