Abstract The leaves of Lippia origanoides Kunth are used in culinary as flavoring regional dishes and remedy for gastrointestinal and respiratory diseases in the Amazon region. The circadian and seasonal study of its essential oil was characterized by GC and GC–MS analysis. The oil components were grouped into monoterpenes, sesquiterpenes and phenylpropanoids, during the dry and rainy season. The main constituents were ( E )-methyl cinnamate, ( E )-nerolidol, p -cymene, 1,8-cineole, carvacrol, α-pinene, ( E )-caryophyllene and γ-terpinene, with great variation throughout the year. In this work, we are reporting the occurrence of a new chemotype for L. origanoides , characterized by an essential oil rich in ( E )-methyl cinnamate and ( E )-nerolidol, with fruity-woody odor, reminiscent of cinnamon, strawberry and wood. The oil yield varied from 1.7% to 4.6%, which is considered a significant value for the production of essential oils on an industrial scale. This new chemotype may have ecological, chemosystematics and taxonomic significance in the management and economic utilization of the species.