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The feasibility of cellulase recycling in the scope of bioethanol production from recycled paper sludge (RPS), an inexpensive byproduct with around 39% of carbohydrates, is analyzed. RPS was easily converted and fermented by enzymes and cells, respectively. Final enzyme partition between solid and liquid phases was investigated, the solid-bound enzymes being efficiently recovered by alkaline washing. RPS hydrolysis and fermentation was conducted over four rounds, recycling the cellulases present in both fractions. A great overall enzyme stability was observed: 71, 64 and 100% of the initial Cel7A, Cel7B and β-glucosidase activities, respectively, were recovered. Even with only 30% of fresh enzymes added on the subsequent rounds, solid conversions of 92, 83 and 71% were achieved for the round 2, 3 and 4, respectively. This strategy enabled an enzyme saving around 53-60%, while can equally contribute to a 40% reduction in RPS disposal costs.
Paper, Saccharomyces cerevisiae Proteins, Ethanol, Hydrolysis, Cellulase recycling, Ultrafiltration, Saccharomyces cerevisiae, Bioreactors, Biofuels, Enzyme Stability, Fermentation, Cellulosic bioethanol, Cellulases, Recycled paper sludge, Recycling, Alkaline wash
Paper, Saccharomyces cerevisiae Proteins, Ethanol, Hydrolysis, Cellulase recycling, Ultrafiltration, Saccharomyces cerevisiae, Bioreactors, Biofuels, Enzyme Stability, Fermentation, Cellulosic bioethanol, Cellulases, Recycled paper sludge, Recycling, Alkaline wash
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