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Bioresource Technology
Article . 2016 . Peer-reviewed
License: Elsevier TDM
Data sources: Crossref
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Valorizing recycled paper sludge by a bioethanol production process with cellulase recycling

Authors: Gomes, Daniel Gonçalves; Domingues, Lucília; Gama, F. M.;

Valorizing recycled paper sludge by a bioethanol production process with cellulase recycling

Abstract

The feasibility of cellulase recycling in the scope of bioethanol production from recycled paper sludge (RPS), an inexpensive byproduct with around 39% of carbohydrates, is analyzed. RPS was easily converted and fermented by enzymes and cells, respectively. Final enzyme partition between solid and liquid phases was investigated, the solid-bound enzymes being efficiently recovered by alkaline washing. RPS hydrolysis and fermentation was conducted over four rounds, recycling the cellulases present in both fractions. A great overall enzyme stability was observed: 71, 64 and 100% of the initial Cel7A, Cel7B and β-glucosidase activities, respectively, were recovered. Even with only 30% of fresh enzymes added on the subsequent rounds, solid conversions of 92, 83 and 71% were achieved for the round 2, 3 and 4, respectively. This strategy enabled an enzyme saving around 53-60%, while can equally contribute to a 40% reduction in RPS disposal costs.

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Portugal
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Keywords

Paper, Saccharomyces cerevisiae Proteins, Ethanol, Hydrolysis, Cellulase recycling, Ultrafiltration, Saccharomyces cerevisiae, Bioreactors, Biofuels, Enzyme Stability, Fermentation, Cellulosic bioethanol, Cellulases, Recycled paper sludge, Recycling, Alkaline wash

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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
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