Paenibacillus polymyxa is categorized as an endospore-forming bacterium and Gram-positive bacteria, which have innate beneficial properties in modern biotechnology application. P. polymyxa extensively reviewed as plant growth promoting bacteria which directly gave benefit to the plants by improving nitrogen fixation from atmosphere, increase phosphorus solubilization and iron acquisition in soil and phytohormone production. This could reduce reliance on chemical fertilizers, which is now a source of environmental conflict and appear to be harmful to human. Therefore, application of P. polymyxa focusing only as functional microbial species in production of biofertilizers. P. polymyxa have been gaining momentum over the last couple of years. The recent discovery in microbial industrial of this bacterium is the production of bioactive compounds like exopolysaccharides (EPS). EPS is not only established as biofilm for the colonization of microbes and act as a sink for the nutrients on plant roots in the rhizosphere. Hence, EPS from P. polymyxa is also useful for health care industries such as disease diagnosis and drug manufacturing. Synthesis of hydrolytic enzymes reported as bioconversion of agricultural wastes that helps to tackle serious environmental problems by creating wealth from waste which can also acts as productive biocontrol agents against pathogens. Hence, P. polymyxa having a wide range of antibacterial metabolites and antifungal compounds, inform of volatile organic compouds, peptides and hydrolytic enzymes, This compounds and biomaterials could be commercially marketed as reliable plant biocontrol agents and pharmaceutical application. Nowadays, researcher extensively reviewed and focused their attention on the potential benefits of P. polymyxa with multiple biological functions that cannot be ignored for human health and wellness.