AbstractThis study aimed to investigate antioxidative activities of various fractions of Abelmoschus manihot leaf extract. The fractions were separated based on polarity and the antioxidant activities of each of them were determined. The leaves were extracted using hexane, acetone and methanol serially. The extract was separated by column chromatography using ethyl acetate-methanol gradient elution as mobile phase and silica gel G-60 as the stationary phase, and resulted in five fractions. The antioxidative activities was determined by DPPH free radical scavenging, ferrous ion chelating, and singlet oxygen quenching. Results showed that the fourth fraction had the highest total phenolics and flavonoids content of 297.43±0.48mg GAE/g extract and 117.31±0.38mg quercetin quivalen/g extract, respectively, and showed the highest antioxidative activities.