Name: Visualization of Mouse Nodal Cilia and Nodal Flow
Creator: Shigenori Nonaka
Keywords: 0301 basic medicine, 0303 health sciences, 03 medical and health sciences, Mice, Microscopy, Animals, Gene Expression Regulation, Developmental, Cilia, Embryo, Mammalian, 10. No inequality

descriptionPublicationkeyboard_double_arrow_right Part of book or chapter of book , Article 01 Jan 2013Publisher:Elsevier BV

Authors: Shigenori Nonaka;

doi: 10.1016/b978-0-12-397944-5.00008-0

pmid: 23522469

Visualization of Mouse Nodal Cilia and Nodal Flow

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Abstract

The earliest left-right asymmetry in mouse development arises in 7.5 days, at the ventral surface of the embryonic node. The node cells possess monocilia beating in rotatory fashion to generate fluid flow from the right to the left (nodal flow). The direction of nodal flow will determine the side of expression of nodal, the responsible gene for "leftness." Nodal flow is visualized by combination of DIC (differential interference contrast) and microbeads in culture medium. Node cilia movement is visualized by DIC, a high-speed camera, and image processing.

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Keywords

Mice, Microscopy, Animals, Gene Expression Regulation, Developmental, Cilia, Embryo, Mammalian, Body Patterning

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